Prolonging the viability of brain slices

The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research. Learn More Here

  • braincubator

    Up to 36 hours


    Save time preparing slices


    Halve the number of animals sacrificed

Current Users

Western Sydney University
University of New South Wales
The University of Sydney
Emory University
Queensland Brain Institute
South Australian Health & Medicine Research Institute
Indiana University
Gedeon Richter Pharmaceutical Company
Vanderbilt University
Ann & Robert Lurie Children’s Hospital of Chicago